Twenty four (24) sexually matured male rabbits between the ages of 5 – 8 months were used to evaluate the effect of Human Menopausal Gonadotrophin (HMG) on the semen characteristic of the matured rabbits bucks. The twenty four male rabbits were divided into four treatment groups identified as: R1M (which served as the control group) R2M, R3M and R4M. Rabbit bucks in R1M (control) were administered with 0.00ml of HMG, R2M group received 0.03ml of HMG, R3M also received 0.06ml of HMG, while group R4M was administered with 0.09ml of HMG injection. The animals were randomly assigned into four treatments of HMG in a completely randomized design (CRD). Each group was further divided three times, giving two animals per replicate. HMG was administered to the animals for three consecutive days. Semen collection from the male reproductive organ of the rabbits was done after five days of administration of the drug. The semen was collected using matured cycling doe to tease the buck making use of an artificial vagina (AV). The semen colour (creamy milky) obtained in this study was similar in all treatment groups. Semen volumes, semen pH, sperm concentration of the semen, live sperm cells values differed significantly between treatment groups and control group. Higher values on semen characteristics were obtained in R4M group. On the other hand the percentage dead sperm cells, sperm motility, and libido levels were significantly (p<0.05) higher in the control group than those in the other treatment groups. This shows that the HMG had a positive influence on the semen characteristics of rabbit bucks. Keywords: Impact, Human Menopausal Gonadotrophin, (Pergonal(r)), Semen Characteristics, Male, Rabbits