The potentials of extract solution of the zobo (Hibiscus sabdariffa ) leaf as an alternative counter stain in Gram staining reactions were studied. The red part of zobo leaves was carefully picked and dried under the sun. The dye was extracted with soxhlet apparatus using methanol as the solvent. After drying to powder, the extract was dissolved in absolute ethanol 10% w/v. The staining solution was used to stain bacteria isolates of E.coli strains, Pseudomonas aeruginosa and Bacillus subtilis. The experimental zobo extracts solution was used with as usual counter stain in place of safranin in Gram staining process. The results obtained therefore, showed that the zobo leave extract can be a substitute to the usual counter stains used in Gram staining reactions, as it clearly differentiated between the Gram positive and Gram negative bacteria. The study has demonstrated the use of dyes from plants as a suitable substitute for staining microorganisms; in a very simple, cheap, easy and readily available procedure. Keywords: Dyes, Plants, Extracts, Staining, Microorganisms.