This study involves evaluation of fatty acid profile, physicochemical, proximate and phytochemical properties of soursop seed oil. The oil was extracted using soxhlet extraction methods with n-hexane. The physicochemical, proximate and phytochemical properties of the soursop seed oil were determined using standard methods, while GC-MS was used to determine the fatty acid profile of the oil. The percentage oil yield was 37.42%. The physicochemical properties of the soursop seed were: specific gravity (0.92), refractive index (1.465), viscosity at 100 oC (3.456 pa s), flash point (200.53 oC), pour point (-15 oC), kinematic viscosity @ 40 oC (8.01 mm2 /s), saponification value (118.9 mg KOH/g oil), acid value (5.61 mg KOH/g oil), iodine value (109.24 gI2/100g oil), peroxide value (2.09 meq O2/kg oil), and pH (5.86). The fatty acid profile of the oil indicated 0.01%, 18.60%, 1.79%, 4.59%, 40.98%, 32.11%, 1.23%, 0.49%), 0.07%, and 0.13% for myristic acid (C14:0), palmit