The aim of this work was to use Lactobacillus as a probiotic in the prevention of gastroenteritis in children. Staphylococcus aureus, Escherichia coli, Salmonella sp and Klebsiella sp were isolated from the different stool samples and identified using cultural characteristics and biochemical tests. Lactobacillus sp was also isolated from yoghurt and identified too. Antimicrobial activity was carried out using disc diffusion method and the zones of inhibition were measured. The cell free supernatants of Lactobacillus sp used as antimicrobial agent inhibited Staphylococcus aureus, Salmonella sp, Klebsiellasp with zones of inhibition of 11mm, 12mm, 10mm respectively while Escherichia coli was not inhibited. The control also had zones of inhibition of 20mm, 29mm, 25mm and 20mm on Staphylococcus aureus, Salmonella sp, Klebsiella sp and Escherichia coli respectively. The intake of probiotics is therefore recommended in the prevention of gastroenteritis in children. Keywo