Lactic acid bacteria isolated from ogiri and soymilk were screened to determine their antibacterial activity against food spoilage organisms. The antimicrobial activity of these probiotic bacteria against many species or microorganisms is one useful property they possess which could be useful to prevent food spoilage caused by certain sensitive bacteria and also to control the speed of propagation of potentially pathogenic bacteria by their application. Lactic acid bacteria are probiotic bacteria that produce metabolites with broader spectrum of activity. The antagonistic properties of these isolates against Escherichia coli, Staphylococcus aureus and Salmonella typhi were examined using agar well diffusion method. Two lactic acid bacterial isolates, namely C1 and C2 were identified as Lactobacillus plantarum and Lactobacillus brevis were effective against all the selected indicator organisms except Salmonella typhi. Among the isolates C1 which has a broader spectrum of activity showed